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dc.rights.licenseAll rights reserveden_US
dc.contributor.advisorNieves Castro, Rafael A.
dc.contributor.authorNieves Ortiz, Miguel A.
dc.date.accessioned2024-10-21T13:31:20Z
dc.date.available2024-10-21T13:31:20Z
dc.date.issued2024
dc.identifier.citationNieves Ortiz, M. A. (2024). Biotechnology Ion Exchange Chromatography Improvement [Unpublished manuscript]. Graduate School, Polytechnic University of Puerto Rico.en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12475/2783
dc.descriptionDesign Project Article for the Graduate Programs at Polytechnic University of Puerto Ricoen_US
dc.description.abstractThis research investigates the influence of different stop collection percentages on ion exchange chromatography in laboratory-scale separations. A direct association was seen between reduced stop collection (percentages of peak max) and increased chromatographic area under the curves, resulting in enhanced substance elution. Four (4) experimental runs showed linear behavior (97%, 95%, 93%, 90%, and 88% peak max). The presence of variability among runs serves to emphasize the susceptibility of outcomes, hence it is important to consider the control of key parameters such as chromatography process conditions and sample stability. The suggestions for future research include (1) adjusting column conditions and (2) investigating causes of variability to enhance reproducibility. This study offers significant contributions to the productivity of a blockbuster drug, by enhancing the uses of ion exchange chromatography. The supporting data was generated using a scaled-down model of the large-scale process operations. Key Terms ⎯Chromatography, Ion Exchange, Scale-Down Model, Stop Collection.en_US
dc.language.isoenen_US
dc.publisherPolytechnic University of Puerto Ricoen_US
dc.relation.ispartofManufacturing Engineering Program;
dc.relation.ispartofseriesSpring-2024;
dc.relation.haspartSan Juanen_US
dc.subject.lcshPolytechnic University of Puerto Rico--Graduate students--Researchen_US
dc.subject.lcshPolytechnic University of Puerto Rico--Graduate students--Postersen_US
dc.subject.lcshPolytechnic University of Puerto Rico--Subject headings--Unassigneden_US
dc.titleBiotechnology Ion Exchange Chromatography Improvementen_US
dc.typeArticleen_US
dc.rights.holderPolytechnic University of Puerto Rico, Graduate Schoolen_US


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