Invalid Assay Reduction Initiative for Silver Stained Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Assays in Quality Control Laboratories
Negrón Vicente, Luis O.
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In this research, the Define, Measure, Analyze, Improve and Control methodology was used to identify and mitigate the common reasons of invalid or unacceptable Silver Stained Polyacrylamide Gel Electrophoresis assays performed in Quality Control Laboratories. The research was focused in the prevention of contaminant bands seen on the gels after staining. It also measured the economic impact to the Quality Control Laboratories operations. Key Terms - Contaminant Bands, Quality Control Laboratories, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), Silver Nitrate Stain for SDS-PAGE.