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dc.rights.licenseAll rights reserveden_US
dc.contributor.advisorNigaglioni, Rolando
dc.contributor.authorValladares Bonilla, Norberto
dc.date.accessioned2022-12-22T11:34:25Z
dc.date.available2022-12-22T11:34:25Z
dc.date.issued2022
dc.identifier.citationValladares Bonilla, N. & Nigaglioni, R. (2022). Evaluation of Residual Protein ELISA Stability of Precoated Plates and Assay Control in a Hight Throughput Environment [Unpublished manuscript]. Graduate School, Polytechnic University of Puerto Rico.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12475/1782
dc.descriptionDesign Project Article for the Graduate Programs at Polytechnic University of Puerto Ricoen_US
dc.description.abstractEvaluation of Enzyme Linked Immunoassay Sorbent Assay (ELISA) overall stability on precoated plates to appraise Residual Protein levels in a wide variety of drug substance samples and filtration process reduces turn around time and give analyst more flexibility on their testing schedule. Results obtained on precoated plates at 5C and -20C showed a positive outcome when the optical densities and the actual titers were compared to the control group. Precoating plates for Residual Protein ELISA demonstrated to be cost-effective, enabling to shorten the turn around time from one week to 72 hours and gives a swifter testing approach towards emergency runs, Process Performance Qualifications submissions, and deviations initiates by the manufacturing departments. Data has shown that freeze thaw mechanism at -80C extend the shelf life of the assay for up to 3 weeks. Key Terms ⎯ ELISA, Freeze Thaw, Residual Protein Stability.en_US
dc.language.isoenen_US
dc.publisherPolytechnic University of Puerto Ricoen_US
dc.relation.ispartofManufacturing Competitiveness;
dc.relation.ispartofseriesFall-2022;
dc.relation.haspartSan Juanen_US
dc.subject.lcshEnzyme-linked immunosorbent assayen_US
dc.subject.lcshPolytechnic University of Puerto Rico--Graduate students--Postersen_US
dc.subject.lcshPolytechnic University of Puerto Rico--Graduate students--Researchen_US
dc.subject.otherFreeze Thaw
dc.subject.otherELISA
dc.titleEvaluation of Residual Protein ELISA Stability of Precoated Plates and Assay Control in a Hight Throughput Environmenten_US
dc.typeArticleen_US
dc.rights.holderPolytechnic University of Puerto Rico, Graduate Schoolen_US


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